![]() To overcome this deficiency there is a push to identify sustainable supplies of both animal and plant protein for human consumption in order to lower greenhouse gas emissions and improve the protein supply chain in agriculture. It is estimated that up to a billion people worldwide, from both developing and developed nations, do not have an adequate intake of protein in their diet which leads to impaired growth and increased risk of disease. SSPs, Seed Storage Protein, Seed Storage Proteins TCA,įile Extension of Applied Biosystems Raw Data Files μg, Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis SSP, Percent Sequence Identity, Pairwise Identity QMEAN, National Center for Biotechnology Infromation PID, Mass Spectrometry, Mass Spectrometer MSA, Liquid Chromatography Coupled to Tandem Mass Spectrometry MF, High Performance Liquid Chromatography IDA, Proton Nuclear Magnetic Resonance 3D-structure,Ĭomplementary Deoxyribonucleic Acid COV,Ĭlustered Regularly Interspaced Short Palindromic Repeats-Associated Protein-9 Nuclease DNA,įast Adaptive Shrinkage Threshold Algorithm FDR, One-Dimensional Sodium Dodecyl Sulfate -Polyacrylamide Gel Electrophoresis 1H NMR, ![]() The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. All other relevant data are within the manuscript and its Supporting information files.įunding: MR received an International Postgraduate Research Scholarship (IPRS) and Australian Postgraduate Award Scholarship (APA) from the Australian Government. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Raw spectral data is available at DOI: 10.25918/data.133. Received: ApAccepted: JPublished: July 9, 2021Ĭopyright: © 2021 Rahman et al. (2021) Shotgun proteomics of Brassica rapa seed proteins identifies vicilin as a major seed storage protein in the mature seed. This study enriches our existing knowledge on rapeseed seed proteins and provides a robust foundation and rational basis for plant bioengineering of seed storage proteins.Ĭitation: Rahman M, Guo Q, Baten A, Mauleon R, Khatun A, Liu L, et al. rapa seed represents the first account of these proteins in the Brassicaceae and analysis indicates high conservation of sequence motifs to other 7S vicilin-like allergenic proteins as well as conservation of major allergenic epitopes in the proteins. The identification of vicilin like proteins in the mature B. ![]() 2S albumin like napin seed storage proteins (SSPs), 11/12S globulin like cruciferin SSPs and 7S globulin like vicilin SSPs were identified in the samples, along with other notable proteins including oil body proteins, namely ten oleosins and two oil body-associated proteins. ![]() Following liquid chromatography tandem mass spectrometry, a total of 34016 spectra were mapped to 323 proteins, where 233 proteins were identified in 3 out of 4 biological replicates by at least 2 unique peptides. To gain insight into the Brassica rapa seed proteome and to confirm the presence and relative quantity of proteins encoded by candidate seed storage genes in the mature seed, shotgun proteomics was carried out on protein extracts from seeds of B. The most abundant proteins reported in the seeds of Brassica are the seed storage proteins cruciferin and napin, belonging to the 12S globulin and 2S albumin families of proteins, respectively. Proteins make up a large percentage of the Brassica seed and are second only to the oil in economic importance with uses for both animal and human nutrition. ![]()
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